1.
Experimental & Molecular Medicine
;
: 240-245, 1998.
Article
in English
| WPRIM
| ID: wpr-159763
ABSTRACT
We studied the regulation of fibronectin (FN) gene expression by cAMP and phorbol-12-myristate-13-acetate (PMA) in HT-1080 human fibrosarcoma cells. Dibutyryl cAMP increased FN synthesis and mRNA levels, while PMA inhibited the cAMP-induced FN synthesis. In transient transfection assays, cAMP increased FN promoter activity, while PMA paradoxically enhanced the cAMP-induced promoter activity. Stable transfection experiments, however, showed that neither cAMP or PMA alone nor together affected FN promoter activity. These results suggest that PMA antagonizes the cAMP-induced FN gene expression and that both the action of cAMP and the inhibition of its action by PMA may occur at the posttranscriptional level in HT-1080 cells.
Subject(s)
Humans , Blotting, Northern , Bucladesine/pharmacology , Bucladesine/antagonists & inhibitors , Enzyme-Linked Immunosorbent Assay , Fibronectins/metabolism , Fibronectins/genetics , Fibrosarcoma/genetics , Gene Expression Regulation , Luciferases/metabolism , Precipitin Tests , Promoter Regions, Genetic , RNA, Messenger/metabolism , Tetradecanoylphorbol Acetate/pharmacology , Transfection , Tumor Cells, Cultured , beta-Galactosidase/metabolism
2.
Indian J Cancer
;
1967 Sep; 4(3): 298-303
Article
in English
| IMSEAR
| ID: sea-50895